Hydroalcoholic Extract of Levisticum officinale Increases cGMP Signaling Pathway by Down-Regulating PDE5 Expression and Induction of Apoptosis in MCF-7 and MDA-MB-468 Breast Cancer Cell Lines

نویسندگان

  • Ali Shahraki Department of Biology, Faculty of Science, University of Sistan and Baluchestan, Zahedan, Iran
  • Marzieh Lotfian Sargazi Cellular and Molecular Research Center Zahedan University of Medical Sciences, Zahedan, Iran
  • Ramin Saravani Cellular and Molecular Research Center Zahedan University of Medical Sciences, Zahedan, Iran
چکیده مقاله:

Background: This study aimed to investigate Levisticum officinale hydroalcoholic extract (LOHE) effect on both cGMP signaling pathway and phosphodiesterase 5 (PDE5) gene expression pattern and to examine the role of LOHE in apoptosis induction of MCF-7 and MDA-MB-468 cell lines. Methods: The half maximal inhibitory concentration (IC50) of LOHE was examined in both cell lines using the MTT assay. Using IC50 values of LOHE on both cells, the type of cell death was detected by flowcytometric analysis.  The values of PDE5 and cGMP were evaluated by real-time PCR and ELISA methods, respectively. Results: The IC50 values were measured as 150 μg/ml for MDA-MB-468 and 200 μg/ml for MCF-7. At 12 hour of treatment, a significant decrease in the PDE5 expression and maximum increase in the amount of intracellular cGMP were observed (p < 0.05). However, these effects were more noticeable in MDA-MB-468 triple-negative cells. Conclusion: Our data suggest that LOHE extract could be a potential source for new strategies towards targeting both PDE5 and cGMP signaling pathways.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Cell Kinetic Study of Tamoxifen Treated MCF-7 and MDA-MB 468 Breast Cancer Cell Lines

Apoptosis could be a major mechanism of antitumor effect of tamoxifen. Therefore this study is designed to characterize the kinetic behavior of tamoxifen-induced apoptosis in the estrogen receptor positive (ER+) and negative (ER-) cell lines, MCF-7 and MDA-MB-468. Frequency of cell death was examined by trypan blue and acridine orange staining. Annexin V-Fluorescein/PI was used in flow cytometr...

متن کامل

cell kinetic study of tamoxifen treated mcf-7 and mda-mb 468 breast cancer cell lines

apoptosis could be a major mechanism of antitumor effect of tamoxifen. therefore this study is designed to characterize the kinetic behavior of tamoxifen-induced apoptosis in the estrogen receptor positive (er+) and negative (er-) cell lines, mcf-7 and mda-mb-468. frequency of cell death was examined by trypan blue and acridine orange staining. annexin v-fluorescein/pi was used in flow cytometr...

متن کامل

Apoptotic potential of two Caryophyllaceae species in MCF-7 and MDA-MB-468 cell lines

Background and objectives: Plants have been used to treat diseases like cancer for many years and today the trend towards their use is increasing. One of the most effective mechanisms of plants against cancer is inducing apoptosis. Apoptosis is a programmed cell death which acts opposite to cell division. It starts in response to some stimuli. Despite the effectiveness of apopt...

متن کامل

Cell Kinetic Study of Tamoxifen Treated MCF-7 and MDA-MB 468 Breast Cancer Cell Lines

Apoptosis could be a major mechanism of antitumor effect of tamoxifen. Therefore this study is designed to characterize the kinetic behavior of tamoxifen-induced apoptosis in the estrogen receptor positive (ER) and negative (ER) cell lines, MCF-7 and MDA-MB-468. Frequency of cell death was examined by trypan blue and acridine orange staining. Annexin V-Fluorescein/PI was used in flow cytometry ...

متن کامل

Ethanolic Extract of Propolis from Kerman Area Triggers Apoptosis and Arrests Cell Cycle in Three Human Breast Cancer Cell Lines MDA-MB-231, SKBR and MCF-7

Background: Cancer is one of the major health problems worldwide and natural resources are being explored to develop anticancer drugs with fewer side effects. Iranian propolis contains components including flavonoids and polyphenols and has various medicinal properties. The aim of this study was to investigate the effect of Ethanolic Extract of Sirch Propolis (EESP) on three br...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ذخیره در منابع من قبلا به منابع من ذحیره شده

{@ msg_add @}


عنوان ژورنال

دوره 23  شماره 4

صفحات  280- 286

تاریخ انتشار 2019-07

با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.

کلمات کلیدی

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023